Recombinant Virus CXCL2/GRO beta/MIP-2/CINC-3 Protein

Name: Recombinant Virus CXCL2/GRO beta/MIP-2/CINC-3 Protein
Brand: Novus Biologicals
SKU: NBP2-35318

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35318

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Product Specifications
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q98157

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL2/GRO beta/MIP-2/CINC-3 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 70 amino acids corresponding to CXCL2/GRO beta/MIP-2/CINC-3 Source: E. coli

Uniprot ID: Q98157

Amino Acid Sequence: LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL2/GRO beta/MIP-2/CINC-3 as determined by LAL method.

Predicted Molecular Mass

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL2 Protein is fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35318

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Viral MIP-2 is one of the three chemokine-like proteins expressed by the human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus, KSHV) and the other is ViMIP-1 and ViMIP-3. It shares 41 % and 48 % with human MIP-1alpha and ViMIP-1, respectively. Viral CXCL2 (MIP-2) has been shown to have antagonist activity towards a wide range of chemokine receptors and has functions of blocking infection by several different human immunodeficiency virus type 1 (HIV-1) strains. It may form part of the response to host defenses contributing to virus-induced neoplasia and may have relevance to KSHV and HIV-I interactions. Additionally, Viral MIP-2 has been shown to activate and chemoattract human eosinphils.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2, Macrophage Inflammatory Protein, Macrophage Inflammatory Protein 2

Gene Symbol

CXCL2

UniProt