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Recombinant Human Cytokeratin 14 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72467

Novus Biologicals, part of Bio-Techne
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NBP1-72467-0.5mg
NBP1-72467-100ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-472 of Human Cytokeratin 14

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

53.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cytokeratin 14 His Protein

Recombinant Human Cytokeratin 14 His Protein

SDS-PAGE: Recombinant Human Cytokeratin 14 His Protein [NBP1-72467] -

SDS-PAGE: Recombinant Human Cytokeratin 14 His Protein [NBP1-72467] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recombinant Human Cytokeratin 14 His Protein

SDS-PAGE: Recombinant Human Cytokeratin 14 His Protein [NBP1-72467] -

SDS-PAGE: Recombinant Human Cytokeratin 14 His Protein [NBP1-72467] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72467
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 14

KRT14, also known as keratin 14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis. Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

CK14, KRT14

Gene Symbol

KRT14

Additional Cytokeratin 14 Products

Product Documents for Recombinant Human Cytokeratin 14 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Cytokeratin 14 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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