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Recombinant Human DDT His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45258

Novus Biologicals, part of Bio-Techne
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NBP1-45258-0.1mg
NBP1-45258-0.5mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-118 of Human DDT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

14.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DDT His Protein

SDS-PAGE: Recombinant Human DDT His Protein [NBP1-45258]

SDS-PAGE: Recombinant Human DDT His Protein [NBP1-45258]

SDS-Page: Recombinant Human DDT His Protein [NBP1-45258] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-45258
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDT

DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

D-Dopachrome Tautomerase

Alternate Names

D-Dopachrome Decarboxylase, DDCT, DOPD, MIF2, Phenylpyruvate Tautomerase II

Gene Symbol

DDT

Additional DDT Products

Product Documents for Recombinant Human DDT His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human DDT His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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