Recombinant Human Desert Hedgehog C23II Protein

Name: Recombinant Human Desert Hedgehog C23II Protein
Brand: Novus Biologicals
SKU: NBP2-34949

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34949

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

O43323

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Desert Hedgehog C23II Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 177 amino acids corresponding to Desert Hedgehog C23II Source: E. coli

Uniprot ID: O43323

Amino Acid Sequence: IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Desert Hedgehog C23II as determined by LAL method.

Predicted Molecular Mass

19.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Desert Hedgehog C23II Protein

SDS-PAGE: Recombinant Human Desert Hedgehog C23II Protein [NBP2-34949]

SDS-Page: Human Desert Hedgehog C23II Protein [NBP2-34949]

Formulation, Preparation and Storage

NBP2-34949

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 10mM PB, pH 6.0, 300mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Desert Hedgehog C23II

Desert hedgehog protein (DHH), also named HHG-3, is belonging to the hedgehog (Hh) family that are highly conserved proteins which are widely represented throughout the animal kingdom. This protein is predicted to be made as a precursor that is autocatalytically cleaved and the biologically active form of Hh molecules corresponds to approximately the N-terminal one half of the precursor molecule. The N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. DHH is essential for a variety of patterning events during development as intercellular signal. It may function as a spermatocyte survival factor in the testes and play an important role in testes development. Mature human DHH shares 97 % amino acid sequence identity with murine DHH.

Gene Symbol

DHH

UniProt