Recombinant Mouse Desert Hedgehog C23II Protein

Name: Recombinant Mouse Desert Hedgehog C23II Protein
Brand: Novus Biologicals
SKU: NBP2-35175

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35175

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q61488

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Desert Hedgehog C23II Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 177 amino acids corresponding to Desert Hedgehog C23II Source: E. coli

Uniprot ID: Q61488

Amino Acid Sequence: IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Desert Hedgehog C23II as determined by LAL method.

Predicted Molecular Mass

20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Desert Hedgehog C23II protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3E1 cells is 5-20 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Desert Hedgehog C23II Protein

SDS-PAGE: Recombinant Mouse Desert Hedgehog C23II Protein [NBP2-35175]

SDS-Page: Mouse Desert Hedgehog C23II Protein [NBP2-35175]

Formulation, Preparation and Storage

NBP2-35175

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Desert Hedgehog C23II

Desert hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are cleaved autocatalytically, and the N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Dhh binds Patched, Patched 2, and Hedgehog interacting protein (Hip), inhibiting the ability of Patched to repress Smoothened signaling. Dhh is produced by Sertoli cells and is required for testis development, Leydig cell differentiation, and spermatogenesis. It is expressed in the female by ovarian granulosa cells and the corporus luteum and is upregulated in ovarian cancer. Dhh is also expressed by Schwann cells and is upregulated following nerve injury.

Gene Symbol

DHH

UniProt