Recombinant Human EIF1B His Protein

Name: Recombinant Human EIF1B His Protein
Brand: Novus Biologicals
SKU: NBP1-51033

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51033

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EIF1B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-113 of Human EIF1B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EIF1B His Protein

SDS-PAGE: Recombinant Human EIF1B His Protein [NBP1-51033]

SDS-Page: Recombinant Human EIF1B Protein [NBP1-51033] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-51033

Formulation	20mM Tris-HCl buffer (pH 8.5), 10% glycerol, 2mM DTT, 0.1M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF1B

EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

eIF1b, eukaryotic translation initiation factor 1B, GC20, Protein translation factor SUI1 homolog GC20, translation factor sui1 homolog

Gene Symbol

EIF1B

Additional EIF1B Products

Product Documents for Recombinant Human EIF1B His Protein

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COA

Product Specific Notices for Recombinant Human EIF1B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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