Recombinant Human Ephrin-B2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72462

Novus Biologicals, part of Bio-Techne
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NBP1-72462-0.5mg
NBP1-72462-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Ephrin-B2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-229 of Human Ephrin-B2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIVLEP IYWNSSNSKF LPGQGLVLYP QIGDKLDIIC PKVDSKTVGQ YEYYKVYMVD KDQADRCTIK KENTPLLNCA KPDQDIKFTI KFQEFSPNLW GLEFQKNKDY YIISTSNGSL EGLDNQEGGV CQTRAMKILM KVGQDASSAG STRNKDPTRR PELEAGTNGR SSTTSPFVKP NPGSSTDGNS AGHSGNNILG SEVALFA

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Ephrin-B2 His Protein

SDS-PAGE: Recombinant Human Ephrin-B2 His Protein [NBP1-72462]

SDS-PAGE: Recombinant Human Ephrin-B2 His Protein [NBP1-72462]

SDS-Page: Recombinant Human Ephrin-B2 His Protein [NBP1-72462] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72462
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 40% glycerol, 200 mM NaCl, 1 mM EDTA
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ephrin-B2

Ephrin-B2 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. It binds to the EPHB4 and EPHA3 receptors. Recombinant human EFNB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Alternate Names

EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1

Gene Symbol

EFNB2

Additional Ephrin-B2 Products

Product Documents for Recombinant Human Ephrin-B2 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ephrin-B2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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