Recombinant Human FABP2/I-FABP Protein

Name: Recombinant Human FABP2/I-FABP Protein
Brand: Novus Biologicals
SKU: NBP2-35004

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35004

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P12104

Conjugate

Unconjugated

Applications

SDS-PAGE

View all FABP2/I-FABP Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 131 amino acids corresponding to FABP2/I-FABP Source: E. coli

Uniprot ID: P12104

Amino Acid Sequence: AFDSTWKVDR SENYDKFMEK MGVNIVKRKL AAHDNLKLTI TQEGNKFTVK ESSAFRNIEV VFELGVTFNY NLADGTELRG TWSLEGNKLI GKFKRTDNGN ELNTVREIIG DELVQTYVYE GVEAKRIFKK D

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of FABP2/I-FABP as determined by LAL method.

Predicted Molecular Mass

15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FABP2/I-FABP Protein

SDS-PAGE: Recombinant Human FABP2/I-FABP Protein [NBP2-35004]

SDS-Page: Human FABP2/I-FABP Protein [NBP2-35004]

Formulation, Preparation and Storage

NBP2-35004

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FABP2/I-FABP

FABP (FABP2) also named intestinal-type fatty acid-binding protein is belonging to the FABPs family and it is encoded by the FABP2 gene in human. The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. Levels of fatty-acid-binding protein have been shown to decline with ageing in the mouse brain, possibly contributing to age-associated decline in synaptic activity. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. Human FABP (FABP2) shares 78 %, 82 %, and 86 % amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Gene Symbol

FABP2

UniProt