Is this product suitable for cell culture study?

Name: Recombinant Human FGF-19 Protein
Brand: Novus Biologicals
SKU: NBP2-35013

Protein NBP2-35013 is validated for functional activity. It has also been endotoxin tested (Less than 1 EU/ug of endotoxin as determined by LAL method), so this protein would be a good product for cell culture study.

Recombinant Human FGF-19 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35013

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O95750

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-19 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 195 amino acids corresponding to FGF-19 Source: E. coli

Uniprot ID: O95750

Amino Acid Sequence: MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-19 as determined by LAL method.

Predicted Molecular Mass

21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF-19 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 x 10^3 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FGF-19 Protein

SDS-PAGE: Recombinant Human FGF-19 Protein [NBP2-35013]

SDS-Page: Recombinant Human FGF-19 Protein [NBP2-35013]

Formulation, Preparation and Storage

NBP2-35013

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-19

Human FGF-19 is encoded by the FGF19 gene. FGF-19 belongs to the FGF-19 subfamily which has three members FGF-19, 21, 23. FGFs are classically considered to be paracrine factors and are known for their roles in tissue patterning and organogenesis during embryogenesis. By contrast, the FGF-19 subfamily has recently been shown to function in an endocrine manner. Members of this subfamily have poor ability of binding to heparin binding site which is a crucial factor in ligand-receptor complex formation. Beta-Klotho has been identified as co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligandreceptor affinity. Unlike most FGFs which bind to and activate more than one FGF receptor, FGF19 is a specific ligand for FGF R4. In FGF-19 transgenic mice, reducing liver triglycerides, increasing fatty acid oxidation, reducing glucose levels and improving insulin sensitivity can be observed.

Long Name

Fibroblast Growth Factor 19

Alternate Names

FGF19

Gene Symbol