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Recombinant Human FHIT His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44387

Novus Biologicals, part of Bio-Techne
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NBP1-44387

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-147 of Human FHIT

Source: E.coli

Amino Acid Sequence: MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FHIT His Protein

SDS-PAGE: Recombinant Human FHIT His Protein [NBP1-44387]

SDS-PAGE: Recombinant Human FHIT His Protein [NBP1-44387]

SDS-Page: Recombinant Human FHIT Protein [NBP1-44387] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-44387
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FHIT

FHIT (fragile histidine triad) is an enzyme that cleaves adenosine 5' PPP 5' A to yield AMP and ADP. FHIT gene encompasses the common fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. This protein physically associates with ubiquitin conjugating enzyme 9. Recombinant human FHIT, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

AP3A hydrolase, AP3AaseFRA3Bbis(5'-adenosyl)-triphosphatase, Diadenosine 5'-5'''-P1, Dinucleosidetriphosphatase, EC 3.6.1.29, fragile histidine triad gene, Fragile histidine triad protein, P3-triphosphate hydrolase, tumor suppressor protein

Gene Symbol

FHIT

Additional FHIT Products

Product Documents for Recombinant Human FHIT His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human FHIT His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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