Could you let us know the MW and concentration of this recombinant? (concentration was not shown on the vial label)

The molecular weight for NBP1-72505 is 14.2 kDa (not including the His tag ~26kDa) and the concentration is 0.25mg/mL.

I was wondering whether the product is suitable for any kind of assay/experiment since the only use suggested is PAGE?

To answer your question regarding the applications FKBP1B Protein (NBP1-72505) can be used for, it has already been tested for its ability to cleave suc-AAFP-pNA but our lab suspects it can be used in other functional assays, although it hasn't been confirmed yet. For untested applications we recommend buying smaller amounts first.

Recombinant Human FKBP12.6 His Protein

Name: Recombinant Human FKBP12.6 His Protein
Brand: Novus Biologicals
SKU: NBP1-72505

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72505

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all FKBP12.6 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-108 of Human FKBP12.6

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FKBP12.6 His Protein

SDS-PAGE: Recombinant Human FKBP12.6 His Protein [NBP1-72505]

SDS-Page: Recombinant Human FKBP12.6 Protein [NBP1-72505]

Formulation, Preparation and Storage

NBP1-72505

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FKBP12.6

FKBP1B, also known as peptidyl-prolyl cis-trans isomerase FKBP1B, is a member of the immunophilin protein family which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus). It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. Recombinant human FKBP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

12.6 kDa FK506 Binding Protein

Alternate Names

FKBP1B, FKBP1L, FKBP9, OTK4

Gene Symbol

FKBP1B

Additional FKBP12.6 Products

Product Documents for Recombinant Human FKBP12.6 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human FKBP12.6 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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