Recombinant Rhesus Macaque Flt-3 Ligand/FLT3L Protein

Name: Recombinant Rhesus Macaque Flt-3 Ligand/FLT3L Protein
Brand: Novus Biologicals
SKU: NBP2-35284

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35284

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Flt-3 Ligand/FLT3L Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 159 amino acids corresponding to Flt-3 Ligand/FLT3L Source: E. coli

Uniprot ID: H9Z6V7

Amino Acid Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQD EELCGALWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQHP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPRSPGALEA TALTAPQRP

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Flt-3 Ligand/FLT3L as determined by LAL method.

Predicted Molecular Mass

18.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Flt3 ligand Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rhesus Macaque Flt-3 Ligand/FLT3L Protein

SDS-PAGE: Recombinant Rhesus Macaque Flt-3 Ligand/FLT3L Protein [NBP2-35284]

SDS-Page: Primate Flt-3 Ligand Protein [NBP2-35284]

Formulation, Preparation and Storage

NBP2-35284

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand promotes the differentiation of multiple hematopoietic cell lineages. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells, B cell differentiation, NK cell development, and myeloid differentiation of hematopoietic stem cells. The mouse and human proteins show cross-species activity.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Gene Symbol

FLT3LG

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Recombinant Rhesus Macaque Flt-3 Ligand/FLT3L Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rhesus Macaque Flt-3 Ligand/FLT3L Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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