Recombinant Rhesus Macaque G-CSF Protein

Name: Recombinant Rhesus Macaque G-CSF Protein
Brand: Novus Biologicals
SKU: NBP2-35297

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35297

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all G-CSF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to G-CSF Source: E. coli

Uniprot ID: F7H1Q6

Amino Acid Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL RHSLGIPWAP LSSCPSQALQ LTGCLSQLHS SLFLYQGLLQ ALEGISPELS PTLDTLQLDI ADFATTIWQQ MEDLGMAPAL QPTQGAMPAF TSAFQRRAGG VLVASHLQRF LELAYRVLRH LAQS

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of G-CSF as determined by LAL method.

Predicted Molecular Mass

18.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

G-CSF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rhesus Macaque G-CSF Protein

SDS-PAGE: Recombinant Rhesus Macaque G-CSF Protein [NBP2-35297]

SDS-Page: Primate G-CSF Protein [NBP2-35297]

Formulation, Preparation and Storage

NBP2-35297

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: G-CSF

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine and mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-alpha activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition it may function in some adhesion or recognition events at the cell surface.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Gene Symbol

CSF3

Additional G-CSF Products

Product Documents for Recombinant Rhesus Macaque G-CSF Protein

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COA

Product Specific Notices for Recombinant Rhesus Macaque G-CSF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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