Recombinant Human gamma-glutamyl hydrolase His Protein

Name: Recombinant Human gamma-glutamyl hydrolase His Protein
Brand: Novus Biologicals
SKU: NBP1-78898

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78898

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all gamma-glutamyl hydrolase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-318 of Human gamma-glutamyl hydrolase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVP VRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFD DGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTAN FHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPN AVKTAFYLAE FFVNEARKNN HHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human gamma-glutamyl hydrolase His Protein

SDS-PAGE: Recombinant Human gamma-glutamyl hydrolase His Protein [NBP1-78898]

SDS-Page: Recombinant Human gamma-glutamyl hydrolase Protein [NBP1-78898]

Formulation, Preparation and Storage

NBP1-78898

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma-glutamyl hydrolase

Gamma-glutamyl hydrolase, also known as GGH, is a homodimeric protein that catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates and is a central enzyme in folyl and antifolyl poly-gamma-glutamate metabolism. GGH may play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Recombinant human GGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Conjugase, EC 3.4.19.9, gamma-glutamyl hydrolase, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase), Gamma-Glu-X carboxypeptidase, GH

Gene Symbol

GGH

Additional gamma-glutamyl hydrolase Products

Product Documents for Recombinant Human gamma-glutamyl hydrolase His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human gamma-glutamyl hydrolase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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