Recombinant Human GDNF Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34888
Key Product Details
Product Specifications
Description
A homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains corresponding to GDNF Source: E. coli
Uniprot ID: P39905
Amino Acid Sequence: SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of GDNF as determined by LAL method.
Predicted Molecular Mass
30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 x 10^7 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human GDNF Protein
![SDS-PAGE: Recombinant Human GDNF Protein [NBP2-34888]](https://resources.bio-techne.com/images/products/Human-GDNF-Protein-SDS-Page-NBP2-34888-img0001.jpg "SDS-PAGE: Recombinant Human GDNF Protein [NBP2-34888]")
SDS-PAGE: Recombinant Human GDNF Protein [NBP2-34888]
SDS-Page: Human GDNF Protein [NBP2-34888]Formulation, Preparation and Storage
NBP2-34888
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4, with 0.05 % Tween-20. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: GDNF
Long Name
Glial Cell line-derived Growth Factor
Alternate Names
ATF, HFB1-GDNF, HGDNF, HSCR3
Gene Symbol
GDNF
UniProt
Additional GDNF Products
Product Documents for Recombinant Human GDNF Protein
Product Specific Notices for Recombinant Human GDNF Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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