Recombinant Hepatitis C Virus His Protein

Name: Recombinant Hepatitis C Virus His Protein
Brand: Novus Biologicals
SKU: NBP1-41149

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41149

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Hepatitis C Virus Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1225-1456 of HCV Hepatitis C Virus

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

27.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Hepatitis C Virus His Protein

SDS-PAGE: Recombinant Hepatitis C Virus His Protein [NBP1-41149]

SDS-Page: Hepatitis C Virus Protein [NBP1-41149] - HCV NS3, 27.9 kDa (268aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-41149

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hepatitis C Virus

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

HCV NS5, Hepatitis C virus non structural protein 5, Hepatitis C virus nonstructural protein 5, Hepatitis C Virus NS5

Additional Hepatitis C Virus Products

Product Documents for Recombinant Hepatitis C Virus His Protein

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COA

Product Specific Notices for Recombinant Hepatitis C Virus His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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