Recombinant Human HMGB1/HMG-1 His (C-Term) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34960
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 223 amino acids corresponding to HMGB1/HMG-1 with 6x His at C-terminus Source: E. coli
Uniprot ID: P09429
Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of HMGB1/HMG-1 as determined by LAL method.
Predicted Molecular Mass
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human HMGB1/HMG-1 His (C-Term) Protein
![SDS-PAGE: Recombinant Human HMGB1/HMG-1 His (C-Term) Protein [NBP2-34960]](https://resources.bio-techne.com/images/products/Human-HMGB1-HMG-1-His-C-Term-Protein-SDS-Page-NBP2-34960-img0001.jpg "SDS-PAGE: Recombinant Human HMGB1/HMG-1 His (C-Term) Protein [NBP2-34960]")
SDS-PAGE: Recombinant Human HMGB1/HMG-1 His (C-Term) Protein [NBP2-34960]
SDS-Page: Human HMGB1/HMG-1 His (C-Term) Protein [NBP2-34960]Formulation, Preparation and Storage
NBP2-34960
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: HMGB1/HMG-1
Long Name
High Mobility Group Protein 1
Alternate Names
HMG-1, HMG1
Gene Symbol
HMGB1
UniProt
Additional HMGB1/HMG-1 Products
Product Documents for Recombinant Human HMGB1/HMG-1 His (C-Term) Protein
Product Specific Notices for Recombinant Human HMGB1/HMG-1 His (C-Term) Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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