Recombinant E. coli HSP10/EPF Protein

Name: Recombinant E. coli HSP10/EPF Protein
Brand: Novus Biologicals
SKU: NBC1-18354

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18354

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HSP10/EPF Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-97 of E.coli HSP10/EPF

Source: E.coli

Amino Acid Sequence: MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

10.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli HSP10/EPF Protein

SDS-PAGE: Recombinant E. coli HSP10/EPF Protein [NBC1-18354]

SDS-Page: Hsp10 Protein [NBC1-18354] - GroES, 10.4kDa (97aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18354

Formulation	25 mM Tris-HCl buffer (pH7.5) 100 mM NaCl, 5mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP10/EPF

GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.

Long Name

Heat Shock 10 kDa Protein 1

Alternate Names

Chaperonin 10 Homolog, EPF, GroES, HSPE1

Gene Symbol

HSPE1

Additional HSP10/EPF Products

Product Documents for Recombinant E. coli HSP10/EPF Protein

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COA

Product Specific Notices for Recombinant E. coli HSP10/EPF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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