Recombinant E. coli HSP70/HSPA1A Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18358

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HSP70/HSPA1A Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-384 of E.coli HSP70/HSPA1A

Source: E.coli

Amino Acid Sequence: MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli HSP70/HSPA1A Protein

SDS-PAGE: Recombinant E. coli HSP70/HSPA1A Protein [NBC1-18358]

SDS-PAGE: Recombinant E. coli HSP70/HSPA1A Protein [NBC1-18358]

SDS-Page: Hsp70 Protein [NBC1-18358] - Dna K, 41.6kDa (384aa) with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18358
Formulation 25 mM Tris-HCl buffer (pH7.5), 100 mM NaCl, 5 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK(amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain(residues385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for Recombinant E. coli HSP70/HSPA1A Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli HSP70/HSPA1A Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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