Human PDGF Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 120-HD

R&D Systems, part of Bio-Techne
Discontinued Product
120-HD has been discontinued. An alternative/replacement product is available: 222-AB. View all PDGF products.

Key Product Details

Source

Human Platelets

Structure / Form

Mostly disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PDGF Proteins and Enzymes »

Product Specifications

Source

Human platelet-derived PDGF protein
The human platelets used for the isolation of this product was certified by the supplier to be HIV 1/2 Ab, HCV Ab, HTLV 1/2, HBc Ab and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

Multiple bands between 28-31 kDa, non-reducing conditions

Activity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 1.5-6 ng/mL.

Formulation, Preparation and Storage

120-HD
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute 1 µg vials at 10 µg/mL in sterile 4 mM HCl. Reconstitute 5 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers.

Two distinct PDGF receptors, the alpha-receptor and the beta-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha-receptor binds both the A and B chains with high affinity whereas the beta-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Long Name

Platelet-derived Growth Factor

Alternate Names

PDGF-1, PDGF1, PDGFA

Entrez Gene IDs

5154 (Human)

Gene Symbol

PDGFA

Additional PDGF Products

Product Documents for Human PDGF Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PDGF Protein, CF

For research use only

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