Recombinant Human IFN-alpha 1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35888

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

P01562

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IFN-alpha 1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 167 amino acids corresponding to IFN-alpha 1 Source: E. coli

Uniprot ID: P01562

Amino Acid Sequence: MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IFN-alpha 1 as determined by LAL method.

Predicted Molecular Mass

19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IFN-alpha 1 protein is fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 x 10^8 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IFN-alpha 1 Protein

SDS-PAGE: Recombinant Human IFN-alpha 1 Protein [NBP2-35888]

SDS-PAGE: Recombinant Human IFN-alpha 1 Protein [NBP2-35888]

SDS-Page: Human Interferon alpha 1a Protein [NBP2-35888]

Formulation, Preparation and Storage

NBP2-35888
Formulation Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, containing 3 % Mannitol, 0.05 % Tween-80.
Preservative 5% Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IFN-alpha 1

Interferon alpha-1, also named interferon alpha-D, is belonging to the type I interferon family and is encoded by the IFNA1gene in humans. Interferon alpha-1 is expressed in peripheral blood leukocytes and lymphoblastoid cells. IFNalpha-1 contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. It plays an important role in inducing non-specific resistance against a broad range of viral infections, and also affects cell proliferation and modulates immune responses. IFNalpha-1a and IFNalpha-1b are two forms of interferon alpha-1 except for the substitution of alanine for valine at position 137. Interferon-alpha represents a group of related but distinct proteins that share over 95 % amino acid sequence homology.

Long Name

Interferon alpha 1

Alternate Names

IFNA1, IFNalpha 1, 1

Gene Symbol

IFNA1

UniProt

P01562 (Human)

Additional IFN-alpha 1 Products

Product Documents for Recombinant Human IFN-alpha 1 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human IFN-alpha 1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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