Recombinant Human IGFBP-rp1/IGFBP-7 Protein

Name: Recombinant Human IGFBP-rp1/IGFBP-7 Protein
Brand: Novus Biologicals
SKU: NBP2-35007

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35007

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q16270

Conjugate

Unconjugated

Applications

SDS-PAGE

View all IGFBP-rp1/IGFBP-7 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 256 amino acids corresponding to IGFBP-rp1/IGFBP-7 Source: E. coli

Uniprot ID: Q16270

Amino Acid Sequence: SSSDTCGPCE PASCPPLPPL GCLLGETRDA CGCCPMCARG EGEPCGGGGA GRGYCAPGME CVKSRKRRKG KAGAAAGGPG VSGVCVCKSR YPVCGSDGTT YPSGCQLRAA SQRAESRGEK AITQVSKGTC EQGPSIVTPP KDIWNVTGAQ VYLSCEVIGI PTPVLIWNKV KRGHYGVQRT ELLPGDRDNL AIQTRGGPEK HEVTGWVLVS PLSKEDAGEY ECHASNSQGQ ASASAKITVV DALHEIPVKK GEGAEL

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IGFBP-rp1/IGFBP-7 as determined by LAL method.

Predicted Molecular Mass

26.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGFBP-rp1/IGFBP-7 Protein

SDS-PAGE: Recombinant Human IGFBP-rp1/IGFBP-7 Protein [NBP2-35007]

SDS-Page: Human IGFBP-rp1/IGFBP-7 Protein [NBP2-35007]

Formulation, Preparation and Storage

NBP2-35007

Preparation Method	This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGFBP-rp1/IGFBP-7

IGF-BP7, also named IBP-7, Mac25 and IGFBPrP1, is belonging to the superfamily of insulin-like growth factor (IGF) binding proteins and is encoded by the igfbp7 gene in human. It is expressed in a wide range of normal human tissues and it generally shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin. IGF-BP7 includes conserved cysteine residues. IGF-BP7 modulates the biological activities of IGF proteins. It also suppresses growth and colony formation of prostate and breast cancer cell lines. Above all, IGF-BP7 is a very important factor in skeletal myogenesis. Human IGF-BP7 cDNA encodes 282 amino acid (a.a.) residue precursor protein with a putative 26 a.a. signal peptide. Human and murine IGF-BP7 share 94% a.a. sequence identity

Long Name

Insulin-like Growth Factor Binding Protein Related Protein 1

Alternate Names

IGFBP-7, IGFBP7, IGFBPrp1, Mac25, PSF

Gene Symbol

IGFBP7

UniProt