Recombinant Human IGFBP-rp1/IGFBP-7 Protein

Name: Recombinant Human IGFBP-rp1/IGFBP-7 Protein
Brand: Novus Biologicals
SKU: NBP2-35007

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35007

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q16270

Conjugate

Unconjugated

Applications

SDS-PAGE

View all IGFBP-rp1/IGFBP-7 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 256 amino acids corresponding to IGFBP-rp1/IGFBP-7 Source: E. coli

Uniprot ID: Q16270

Amino Acid Sequence: SSSDTCGPCE PASCPPLPPL GCLLGETRDA CGCCPMCARG EGEPCGGGGA GRGYCAPGME CVKSRKRRKG KAGAAAGGPG VSGVCVCKSR YPVCGSDGTT YPSGCQLRAA SQRAESRGEK AITQVSKGTC EQGPSIVTPP KDIWNVTGAQ VYLSCEVIGI PTPVLIWNKV KRGHYGVQRT ELLPGDRDNL AIQTRGGPEK HEVTGWVLVS PLSKEDAGEY ECHASNSQGQ ASASAKITVV DALHEIPVKK GEGAEL

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IGFBP-rp1/IGFBP-7 as determined by LAL method.

Predicted Molecular Mass

26.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IGFBP-rp1/IGFBP-7 Protein

SDS-PAGE: Recombinant Human IGFBP-rp1/IGFBP-7 Protein [NBP2-35007]

SDS-Page: Human IGFBP-rp1/IGFBP-7 Protein [NBP2-35007]

Formulation, Preparation and Storage

NBP2-35007

Preparation Method	This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IGFBP-rp1/IGFBP-7

IGF-BP7, also named IBP-7, Mac25 and IGFBPrP1, is belonging to the superfamily of insulin-like growth factor (IGF) binding proteins and is encoded by the igfbp7 gene in human. It is expressed in a wide range of normal human tissues and it generally shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin. IGF-BP7 includes conserved cysteine residues. IGF-BP7 modulates the biological activities of IGF proteins. It also suppresses growth and colony formation of prostate and breast cancer cell lines. Above all, IGF-BP7 is a very important factor in skeletal myogenesis. Human IGF-BP7 cDNA encodes 282 amino acid (a.a.) residue precursor protein with a putative 26 a.a. signal peptide. Human and murine IGF-BP7 share 94% a.a. sequence identity

Long Name

Insulin-like Growth Factor Binding Protein Related Protein 1

Alternate Names

IGFBP-7, IGFBP7, IGFBPrp1, Mac25, PSF

Gene Symbol

IGFBP7

UniProt