Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35305
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 159 amino acids corresponding to IL-1 alpha/IL-1F1 Source: E. coli
Uniprot ID: P48089
Amino Acid Sequence: SAPFSFLSNM TYHFIRIIKH EFILNDTLNQ TIIRANDQHL TAAAIHNLDE AVKFDMGAYT SSKDDTKVPV ILRISKTQLY VSAQDEDQPV LLKEMPEINK TITGSETNFL FFWETHGTKN YFISVAHPNL FIATKHDNWV CLAKGLPSIT DFQILENQA
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of IL-1 alpha/IL-1F1 as determined by LAL method.
Predicted Molecular Mass
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL1 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein
![SDS-PAGE: Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein [NBP2-35305]](https://resources.bio-techne.com/images/products/Primate-IL-1-alpha-IL-1F1-Protein-SDS-Page-NBP2-35305-img0001.jpg "SDS-PAGE: Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein [NBP2-35305]")
SDS-PAGE: Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein [NBP2-35305]
SDS-Page: Primate IL-1 alpha/IL-1F1 Protein [NBP2-35305]Formulation, Preparation and Storage
NBP2-35305
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-1 alpha/IL-1F1
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Gene Symbol
IL1A
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein
Product Specific Notices for Recombinant Rhesus Macaque IL-1 alpha/IL-1F1 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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