Recombinant Mouse IL-13 Protein

Name: Recombinant Mouse IL-13 Protein
Brand: Novus Biologicals
SKU: NBP2-35103

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35103

Submit a review

Citations (1)

Product Datasheet / COA

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P20109

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-13 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 111 amino acids corresponding to IL-13 Source: E. coli

Uniprot ID: P20109

Amino Acid Sequence: MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-13 as determined by LAL method.

Predicted Molecular Mass

12.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-13 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 x 10^5 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-13 Protein

SDS-PAGE: Recombinant Mouse IL-13 Protein [NBP2-35103]

SDS-Page: Mouse IL-13 Protein [NBP2-35103]

Formulation, Preparation and Storage

NBP2-35103

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-13

Murine Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 11 and secreted by many cell types, especially T helper type 2 (Th2) cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 has been implicated as a key factor in asthma, allergy, atopy and inflammatory response, establishing the protein as a valuable therapeutic target. Human, mouse and rat IL-3 share low homology, but have cross species activity.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Gene Symbol

IL13

UniProt