Recombinant Human IL-16 Protein

Name: Recombinant Human IL-16 Protein
Brand: Novus Biologicals
SKU: NBP2-35032

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35032

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q14005

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-16 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 130 amino acids corresponding to IL-16 Source: E. coli

Uniprot ID: Q14005

Amino Acid Sequence: MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-16, 130 a.a. as determined by LAL method.

Predicted Molecular Mass

13.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-16 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CD4+ T Lymphocytes is in a concentration range of 1.0-100 ng/ml

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-16 Protein

SDS-PAGE: Recombinant Human IL-16 Protein [NBP2-35032]

SDS-Page: Human IL-16 130 a.a Protein [NBP2-35032]

Formulation, Preparation and Storage

NBP2-35032

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-16

Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2R alpha on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16(130 a.a.) contains 130 amino acid residues and it shares 85% a.a. sequence identity with murine IL-16.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Gene Symbol

IL16

UniProt