Recombinant Mouse IL-16 Protein

Name: Recombinant Mouse IL-16 Protein
Brand: Novus Biologicals
SKU: NBP2-35104

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35104

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O54824

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-16 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 118 amino acids corresponding to IL-16 Source: E. coli

Uniprot ID: O54824

Amino Acid Sequence: SAASASAASD ISVESKEATV CTVTLEKTSA GLGFSLEGGK GSLHGDKPLT INRIFKGTEQ GEMVQPGDEI LQLAGTAVQG LTRFEAWNVI KALPDGPVTI VIRRTSLQCK QTTASADS

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-16 as determined by LAL method.

Predicted Molecular Mass

12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL16 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-16 Protein

SDS-PAGE: Recombinant Mouse IL-16 Protein [NBP2-35104]

SDS-Page: Mouse IL-16 121 a.a Protein [NBP2-35104]

Formulation, Preparation and Storage

NBP2-35104

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-16

Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Ralpha on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant murine interleukin-16 contains 118 amino acid residues and it shares about 85 % a.a. sequence identity with different isoforms of human IL-16.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Gene Symbol

IL16

UniProt