Recombinant Human IL-17B Protein

Name: Recombinant Human IL-17B Protein
Brand: Novus Biologicals
SKU: NBP2-35044

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35044

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9UHF5

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-17B Proteins and Enzymes »

Product Specifications

Description

A non-disulfide-linked homodimer of two 161 amino acid polypeptide chains corresponding to IL-17B Source: E. coli

Uniprot ID: Q9UHF5

Amino Acid Sequence: MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-17B as determined by LAL method.

Predicted Molecular Mass

36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-17B protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion of human HepG2 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-17B Protein

SDS-PAGE: Recombinant Human IL-17B Protein [NBP2-35044]

SDS-Page: Human IL-17B Protein [NBP2-35044]

Formulation, Preparation and Storage

NBP2-35044

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.1 % Tween-20 and 3 % Trehalose.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-17B

IL-17B, also named IL20, NIRF and ZCYTO7, is belonging to the IL-17 family and it stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. IL-17B mRNA is expressed in adult pancreas, small intestine, and stomach. The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.

Long Name

Interleukin 17B

Alternate Names

IL17B

Gene Symbol