Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34937
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1ra/IL-1F3/IL1RN Source: E. coli
Uniprot ID: O18999
Amino Acid Sequence: HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-1ra/IL-1F3/IL1RN as determined by LAL method.
Predicted Molecular Mass
17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 3.0 ug/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1alpha.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein
![SDS-PAGE: Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]](https://resources.bio-techne.com/images/products/Equine-IL-1ra-IL-1F3-IL1RN-Protein-SDS-Page-NBP2-34937-img0001.jpg "SDS-PAGE: Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]")
SDS-PAGE: Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]
SDS-Page: Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]Formulation, Preparation and Storage
NBP2-34937
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-1ra/IL-1F3
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
UniProt
Additional IL-1ra/IL-1F3 Products
Product Documents for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein
Product Specific Notices for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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