Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein

Name: Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein
Brand: Novus Biologicals
SKU: NBP2-34937

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34937

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O18999

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-1ra/IL-1F3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1ra/IL-1F3/IL1RN Source: E. coli

Uniprot ID: O18999

Amino Acid Sequence: HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-1ra/IL-1F3/IL1RN as determined by LAL method.

Predicted Molecular Mass

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 3.0 ug/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1alpha.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein

SDS-PAGE: Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]

SDS-Page: Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]

Formulation, Preparation and Storage

NBP2-34937

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-1ra/IL-1F3

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. It is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-1RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The equus caballus IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1beta and 19 % homology to IL-1alpha. It also shares 78 %~80 % a.a. sequence identity with murine, rat, porcine IL-1RA.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Gene Symbol

IL1RN

UniProt