Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein

Name: Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein
Brand: Novus Biologicals
SKU: NBP2-35225

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35225

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-1ra/IL-1F3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1ra/IL-1F3/IL1RN Source: E. coli

Uniprot ID: P25086

Amino Acid Sequence: HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-1ra/IL-1F3/IL1RN as determined by LAL method.

Predicted Molecular Mass

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-1ra/IL-1F3/IL1RN protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1 alpha-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 x 10^3 IU/mg in the presence of 50 pg/ml IL-1ra/IL-1F3/IL1RN

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein

SDS-PAGE: Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein [NBP2-35225]

SDS-Page: Rat IL-1ra/IL-1F3/IL1RN Protein [NBP2-35225]

Formulation, Preparation and Storage

NBP2-35225

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-1ra/IL-1F3

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL- 1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89 % and 73 % a.a. sequence homology with murine and human IL-1RA.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Gene Symbol

IL1RN

Additional IL-1ra/IL-1F3 Products

Product Documents for Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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