Recombinant Human IL-1ra/IL-1F3/IL1RN Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35894
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1ra/IL-1F3/IL1RN Source: E. coli
Uniprot ID: P18510
Amino Acid Sequence: RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-1ra/IL-1F3/IL1RN as determined by LAL method.
Predicted Molecular Mass
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL-1ra/IL-1F3/IL1RN protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 x 10^4 IU/mg in the presence of 50 pg/ml rHuIL-1alpha
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human IL-1ra/IL-1F3/IL1RN Protein
![SDS-PAGE: Recombinant Human IL-1ra/IL-1F3/IL1RN Protein [NBP2-35894]](https://resources.bio-techne.com/images/products/Human-IL-1ra-IL-1F3-IL1RN-Protein-SDS-Page-NBP2-35894-img0001.jpg "SDS-PAGE: Recombinant Human IL-1ra/IL-1F3/IL1RN Protein [NBP2-35894]")
SDS-PAGE: Recombinant Human IL-1ra/IL-1F3/IL1RN Protein [NBP2-35894]
SDS-Page: Human IL-1ra/IL-1F3/IL1RN Protein [NBP2-35894]Formulation, Preparation and Storage
NBP2-35894
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-1ra/IL-1F3
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
UniProt
Additional IL-1ra/IL-1F3 Products
Product Documents for Recombinant Human IL-1ra/IL-1F3/IL1RN Protein
Product Specific Notices for Recombinant Human IL-1ra/IL-1F3/IL1RN Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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