Recombinant Human IL-21 Protein

Name: Recombinant Human IL-21 Protein
Brand: Novus Biologicals
SKU: NBP2-35057

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35057

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9HBE4

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-21 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 133 amino acids corresponding to IL-21 Source: E. coli

Uniprot ID: Q9HBE4

Amino Acid Sequence: QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-21 as determined by LAL method.

Predicted Molecular Mass

15.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is less than or equal to 2 ng/mL. The specific activity of recombinant human IL-21 > 5.0 x 10^5 U/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-21 Protein

SDS-PAGE: Recombinant Human IL-21 Protein [NBP2-35057]

SDS-Page: Human IL-21 Protein [NBP2-35057]

Formulation, Preparation and Storage

NBP2-35057

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-21

Human IL-21 is encoded by IL-21 gene located on Chr. 4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

UniProt