Recombinant Mouse IL-21 Protein

Name: Recombinant Mouse IL-21 Protein
Brand: Novus Biologicals
SKU: NBP2-35121

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35121

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Product Datasheet / COA

Catalog #

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9ES17

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-21 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 129 amino acids corresponding to IL-21 Source: E. coli

Uniprot ID: Q9ES17

Amino Acid Sequence: HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-21 as determined by LAL method.

Predicted Molecular Mass

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-21 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 x 10^4 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-21 Protein

SDS-PAGE: Recombinant Mouse IL-21 Protein [NBP2-35121]

SDS-Page: Mouse IL-21 Protein [NBP2-35121]

Formulation, Preparation and Storage

NBP2-35121

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-21

IL-21 is encoded by IL-21 gene located on Chr. 4 in mouse. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. The murine IL-21 precursor contains a predicted 17 amino acid (a.a.) signal sequence and a 129 a.a. mature chain. Mature murine IL-21 shares 66 %, 59 %, 58 % and 88 % a.a. sequence identity with mature canine, human, rabbit and rat IL-21, respectively.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

UniProt