Recombinant Human IL-3 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34863
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 133 amino acids corresponding to IL-3 Source: E. coli
Uniprot ID: P08700
Amino Acid Sequence: APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-3 as determined by LAL method.
Predicted Molecular Mass
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL3 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human IL-3 Protein
![SDS-PAGE: Recombinant Human IL-3 Protein [NBP2-34863]](https://resources.bio-techne.com/images/products/Human-IL-3-Protein-SDS-Page-NBP2-34863-img0001.jpg "SDS-PAGE: Recombinant Human IL-3 Protein [NBP2-34863]")
SDS-PAGE: Recombinant Human IL-3 Protein [NBP2-34863]
SDS-Page: Human IL-3 Protein [NBP2-34863]Formulation, Preparation and Storage
NBP2-34863
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-3
Additional IL-3 Products
Product Documents for Recombinant Human IL-3 Protein
Product Specific Notices for Recombinant Human IL-3 Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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