Recombinant Canine IL-3 Protein

Name: Recombinant Canine IL-3 Protein
Brand: Novus Biologicals
SKU: NBP2-34898

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34898

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

Q9BDX4

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 120 amino acids corresponding to IL-3 Source: E. coli

Uniprot ID: Q9BDX4

Amino Acid Sequence: RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-3 as determined by LAL method.

Predicted Molecular Mass

14.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Canine IL-3 Protein

SDS-PAGE: Recombinant Canine IL-3 Protein [NBP2-34898]

SDS-Page: Canine IL-3 Protein [NBP2-34898]

Formulation, Preparation and Storage

NBP2-34898

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-3

Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The canine IL-3 reported to be a monomer, as it is known, contains 120 amino acids residues which is a single non-glycosylated polypeptide. The canine IL-3 shares < 40 % amino acid sequence identity with IL-3 of other mammals.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Gene Symbol

IL3

UniProt