Recombinant Rhesus Macaque IL-3 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35308
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 124 amino acids corresponding to IL-3 Source: E. coli
Uniprot ID: P25140
Amino Acid Sequence: APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-3 as determined by LAL method.
Predicted Molecular Mass
14.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL-3 protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 x 10^5 IU/mg
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rhesus Macaque IL-3 Protein
![HPLC: Recombinant Rhesus Macaque IL-3 Protein [NBP2-35308]](https://resources.bio-techne.com/images/products/Recombinant-Primate-IL-3-Protein-HPLC-NBP2-35308-img0002.jpg "HPLC: Recombinant Rhesus Macaque IL-3 Protein [NBP2-35308]")
HPLC: Recombinant Rhesus Macaque IL-3 Protein [NBP2-35308]
HPLC: Recombinant Primate IL-3 Protein [NBP2-35308] - Primate IL3 Protein [NBP2-35308]Formulation, Preparation and Storage
NBP2-35308
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at < -20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-3
Long Name
Interleukin 3
Alternate Names
IL3, MCGF, MULTI-CSF
Gene Symbol
IL3
Additional IL-3 Products
Product Documents for Recombinant Rhesus Macaque IL-3 Protein
Product Specific Notices for Recombinant Rhesus Macaque IL-3 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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