Could you let us know the following information? 1) Lyophilized buffer formulation (e.g. Lyophilized from PBS.) 2) Preservative/Concentration

Name: Recombinant Human IL-36 alpha/IL-1F6 Protein
Brand: Novus Biologicals
SKU: NBP2-34880

This protein is supplied in the lyophilized format from the same associated lab of ours, and can be used in the functional assays in vivo (so should not have any preservative and endotoxin-tested. Our lab's databases show that recombinant protein was produce in Escherichia coli with a plasmid containing the gene of interest. It is lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, and no preservative of any kind.

Recombinant Human IL-36 alpha/IL-1F6 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34880

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9UHA7

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-36 alpha/IL-1F6 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 158 amino acids corresponding to IL-36 alpha/IL-1F6 Source: E. coli

Uniprot ID: Q9UHA7

Amino Acid Sequence: MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36 alpha/IL-1F6 as determined by LAL method.

Predicted Molecular Mass

17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL36 alpha Protein is fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36alpha at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-36 alpha/IL-1F6 Protein

SDS-PAGE: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34880]

SDS-Page: Recombinant Human IL-36 alpha/IL-1F6 Protein [NBP2-34880]

Formulation, Preparation and Storage

NBP2-34880

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 alpha/IL-1F6

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on Tcells. Recombinant human interleukin-36 alpha (153 a.a.) contains 153 amino acids residues which is a single non-glycosylated polypeptide and it shares 57 - 68 % a.a. sequence identity with mouse, rabbit, equine and bovine IL-36alpha and 27 - 57 % a.a. sequence identity with other novel IL-1 family members.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Gene Symbol

IL36A

UniProt