Recombinant Human IL-36Ra/IL-1F5 Protein

Name: Recombinant Human IL-36Ra/IL-1F5 Protein
Brand: Novus Biologicals
SKU: NBP2-34872

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34872

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q9UBH0

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-36Ra/IL-1F5 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated biologically active polypeptide chain corresponding to 154 amino acids

Source: E. coli

Amino Acid Sequence: VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36Ra/IL-1F5 as determined by LAL method.

Predicted Molecular Mass

16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL36RA Protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 20 ng/ml of recombinant human IL-36 beta.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-36Ra/IL-1F5 Protein

SDS-PAGE: Recombinant Human IL-36Ra/IL-1F5 Protein [NBP2-34872]

SDS-Page: Human IL-36Ra/IL-1F5 Protein [NBP2-34872]

Formulation, Preparation and Storage

NBP2-34872

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36Ra/IL-1F5

Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes.IL-36RA has been reported to antagonize the biological activity of IL-36alpha (IL-1F6), IL-36beta (IL-1F8), and IL-36gamma (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant murine interleukin-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with human IL-36RA.

Long Name

Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5

Alternate Names

FIL1 delta, IL-1HY1, IL-1L1, IL-1RP3, IL1F5, IL36Ra, IL36RN

Gene Symbol

IL36RN

UniProt