Recombinant Human IL-36Ra/IL-1F5 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34872
Key Product Details
Product Specifications
Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 154 amino acids
Source: E. coli
Amino Acid Sequence: VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-36Ra/IL-1F5 as determined by LAL method.
Predicted Molecular Mass
16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL36RA Protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 20 ng/ml of recombinant human IL-36 beta.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human IL-36Ra/IL-1F5 Protein
![SDS-PAGE: Recombinant Human IL-36Ra/IL-1F5 Protein [NBP2-34872]](https://resources.bio-techne.com/images/products/Human-IL-36Ra-IL-1F5-Protein-SDS-Page-NBP2-34872-img0001.jpg "SDS-PAGE: Recombinant Human IL-36Ra/IL-1F5 Protein [NBP2-34872]")
SDS-PAGE: Recombinant Human IL-36Ra/IL-1F5 Protein [NBP2-34872]
SDS-Page: Human IL-36Ra/IL-1F5 Protein [NBP2-34872]Formulation, Preparation and Storage
NBP2-34872
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-36Ra/IL-1F5
Long Name
Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5
Alternate Names
FIL1 delta, IL-1HY1, IL-1L1, IL-1RP3, IL1F5, IL36Ra, IL36RN
Gene Symbol
IL36RN
UniProt
Additional IL-36Ra/IL-1F5 Products
Product Documents for Recombinant Human IL-36Ra/IL-1F5 Protein
Product Specific Notices for Recombinant Human IL-36Ra/IL-1F5 Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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