Recombinant Rat IL-4 Protein

Name: Recombinant Rat IL-4 Protein
Brand: Novus Biologicals
SKU: NBP2-35252
Rating: 5 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35252

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P20096

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 125 amino acids corresponding to IL-4 Source: E. coli

Uniprot ID: P20096

Amino Acid Sequence: HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-4 as determined by LAL method.

Predicted Molecular Mass

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL4 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-35252 in the following applications:

In vitro bioactivity in cell culture (1 Review)

Scientific Data Images for Recombinant Rat IL-4 Protein

SDS-PAGE: Recombinant Rat IL-4 Protein [NBP2-35252]

SDS-Page: Rat IL-4 Protein [NBP2-35252]

Bioactivity: Recombinant Rat IL-4 Protein [NBP2-35252] - Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6. Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using Image from verified customer review.

Formulation, Preparation and Storage

NBP2-35252

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5% trehalose.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-4

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. IL-4 can enhance both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rat IL-4 shares about 40 % ~ 45 % a.a. sequence identity with human, mouse, rhesus macaque IL-4 and they are species-specific in their activities.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Gene Symbol

IL4

UniProt