Recombinant Mouse IL-5 Protein

Name: Recombinant Mouse IL-5 Protein
Brand: Novus Biologicals
SKU: NBP2-35132

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35132

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P04401

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-5 Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 113 amino acid chains corresponding to IL-5 Source: E. coli

Uniprot ID: P04401

Amino Acid Sequence: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-5 as determined by LAL method.

Predicted Molecular Mass

26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL5 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-5 Protein

SDS-PAGE: Recombinant Mouse IL-5 Protein [NBP2-35132]

SDS-Page: Mouse IL-5 Protein [NBP2-35132]

HPLC: Recombinant Mouse IL-5 Protein [NBP2-35132]

Formulation, Preparation and Storage

NBP2-35132

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, pH 9.0, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-5

IL-5, also named B-cell differentiation factor I, eosinophil differentiation factor and TRF, is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. The cDNA for murine IL-5 encodes a signal peptide and a 113 amino acid mature protein. Mature murine IL-5 shares 70 %, 94 %, 58 %, 66 %, 59 % and 63 % a.a. sequence identity with human, rat, canine, equine, feline and porcine IL5, respectively. It shows cross-reactivity with human IL5 receptor.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

UniProt