Recombinant Mouse IL-7 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35136
Key Product Details
Source
E. coli
Accession #
Conjugate
Unconjugated
Applications
Bioactivity, Flow Cytometry, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 129 amino acids corresponding to IL-7 Source: E. coli
Uniprot ID: Q544C8
Amino Acid Sequence: ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IL-7 as determined by LAL method.
Predicted Molecular Mass
14.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL7 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x 10^6 IU/mg.
Application Notes
Use in Flow reported in scientific literature (PMID:35874724).
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse IL-7 Protein
![SDS-PAGE: Recombinant Mouse IL-7 Protein [NBP2-35136]](https://resources.bio-techne.com/images/products/Mouse-IL-7-Protein-SDS-Page-NBP2-35136-img0001.jpg "SDS-PAGE: Recombinant Mouse IL-7 Protein [NBP2-35136]")
SDS-PAGE: Recombinant Mouse IL-7 Protein [NBP2-35136]
SDS-Page: Mouse IL-7 Protein [NBP2-35136]Formulation, Preparation and Storage
NBP2-35136
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 % trehalose. |
| Preservative | Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IL-7
Additional IL-7 Products
Product Documents for Recombinant Mouse IL-7 Protein
Product Specific Notices for Recombinant Mouse IL-7 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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