Recombinant Human IMP Dehydrogenase 2/IMPDH2 His Protein

Name: Recombinant Human IMP Dehydrogenase 2/IMPDH2 His Protein
Brand: Novus Biologicals
SKU: NBP1-72513

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72513

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all IMP Dehydrogenase 2/IMPDH2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-514 of Human IMP Dehydrogenase 2/IMPDH2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM GSRLVGIISS RDIDFLKEEE HDCFLEEIMT KREDLVVAPA GITLKEANEI LQRSKKGKLP IVNEDDELVA IIARTDLKKN RDYPLASKDA KKQLLCGAAI GTHEDDKYRL DLLAQAGVDV VVLDSSQGNS IFQINMIKYI KDKYPNLQVI GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLACG RPQATAVYKV SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE GGVHSLHSYE KRLF

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IMP Dehydrogenase 2/IMPDH2 His Protein

SDS-PAGE: Recombinant Human IMP Dehydrogenase 2/IMPDH2 His Protein [NBP1-72513]

SDS-Page: Recombinant Human IMP Dehydrogenase 2/IMPDH2 Protein [NBP1-72513] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72513

Formulation	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 150 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IMP Dehydrogenase 2/IMPDH2

IMPDH2 belongs to the IMPDH/GMPR family. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. Also IMPDH1 and IMPDH2 are targets for the important immunosuppressive drug, mycophenolic acid (MPA). Recombinant human IMPDH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Inosine 5'-Monophosphate Dehydrogenase 2

Alternate Names

IMDH2, IMP Oxireductase 2, IMPD2, IMPDH2

Gene Symbol

IMPDH2

Additional IMP Dehydrogenase 2/IMPDH2 Products

Product Documents for Recombinant Human IMP Dehydrogenase 2/IMPDH2 His Protein

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COA

Product Specific Notices for Recombinant Human IMP Dehydrogenase 2/IMPDH2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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