Recombinant Human Indian Hedgehog/Ihh C28II Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35012
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 176 amino acids corresponding to Indian Hedgehog/Ihh Source: E. coli
Uniprot ID: Q14623
Amino Acid Sequence: IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of Indian Hedgehog/Ihh as determined by LAL method.
Predicted Molecular Mass
19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Indian Hedgehog Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ug/ml.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35012
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, 0.02% Tween-20, pH 7.0. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Indian Hedgehog/Ihh
Additional Indian Hedgehog/Ihh Products
Product Documents for Recombinant Human Indian Hedgehog/Ihh C28II Protein
Product Specific Notices for Recombinant Human Indian Hedgehog/Ihh C28II Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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