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Recombinant Human IRGM His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23131

Novus Biologicals, part of Bio-Techne
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NBP2-23131

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 23-181 of Human IRGM

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKETLKIVSR TPVNITMAGD SGNGMSTFIS ALRNTGHEGK ASPPTELVKA TQRCASYFSS HFSNVVLWDL PGTGSATTTL ENYLMEMQFN RYDFIMVASA QFSMNHVMLA KTAEDMGKKF YIVWTKLDMD LSTGALPEVQ LLQIRENVLE NLQKERVCEY

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IRGM His Protein

SDS-PAGE: Recombinant Human IRGM His Protein [NBP2-23131]

SDS-PAGE: Recombinant Human IRGM His Protein [NBP2-23131]

SDS-Page: IRGM Protein [NBP2-23131]

Formulation, Preparation and Storage

NBP2-23131
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRGM

Immunity-related GTPase family M protein, also known as IRGM, is required for clearance of acute protozoan and bacterial infections. This protein functions in innate immune response probably through regulation of autophagy. It may regulate proinflammatory cytokine production and prevent endotoxemia upon infection and may also play a role in macrophages adhesion and motility. Recombinant human IRGM protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

IFI1, immunity-related GTPase family M protein, immunity-related GTPase family, M, interferon-inducible protein 1, IRGM1, LPS-stimulated RAW 264.7 macrophage protein 47 homolog, LRG47, LRG-47, LRG-47-like protein, M1, MGC149263, MGC149264

Gene Symbol

IRGM

Additional IRGM Products

Product Documents for Recombinant Human IRGM His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human IRGM His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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