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LAR/PTPRF Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81325PEP

Novus Biologicals, part of Bio-Techne
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NBP1-81325PEP

Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PTPRF.

Source: E. coli

Amino Acid Sequence: WHPPKELPGELLGYRLQYCRADEARPNTIDFGKDDQHFTVTGLHKGTTYIFRLAAKNRAGLGEEFEKEIRTPEDLPSGFPQNLHVTGLTTSTTELAWDPPVLAERNGRIISYTVVFRDINSQQE

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-81325.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP1-81325PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: LAR

PTPRF, also known as Receptor-type tyrosine-protein phosphatase F, is a 213kDa 1907 amino acid protein with a shorter 212kDa 1898 isoform. PTPRF is a member of the protein tyrosine phosphatase (PTP) family and functions as a possible cell adhesion receptor. Research is being conducted on PTPRF in relation to melanoma, carcinoma, retinitis, thyroid carcinoma, ureterocele, lung cancer, breast cancer and obesity. PTPRF is linked to the cell adhesion and insulin signaling pathways where it interacts with CAV1, PRPF40A, SKIL, ZNF512B and JUP.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Alternate Names

PTPRF

Gene Symbol

PTPRF

Additional LAR Products

Product Documents for LAR/PTPRF Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LAR/PTPRF Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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