Recombinant Human Leptin/OB Protein

Name: Recombinant Human Leptin/OB Protein
Brand: Novus Biologicals
SKU: NBP2-34934

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34934

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P41159

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Leptin/OB Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to Leptin/OB Source: E. coli

Uniprot ID: P41159

Amino Acid Sequence: VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Leptin/OB as determined by LAL method.

Predicted Molecular Mass

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Leptin Protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Leptin/OB Protein

SDS-PAGE: Recombinant Human Leptin/OB Protein [NBP2-34934]

SDS-Page: Human Leptin/OB Protein [NBP2-34934]

Formulation, Preparation and Storage

NBP2-34934

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Leptin/OB

Human Leptin plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. The protein is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus); (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of alpha-MSH, an appetite suppressant. This appetite inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3- 36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity.

Alternate Names

LEP, OB

Gene Symbol

LEP

UniProt