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Recombinant Human LITAF His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23200

Novus Biologicals, part of Bio-Techne
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NBP2-23200

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-161 of Human LITAF

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVPGPYQAA TGPSSAPSAP PSYEETVAVN SYYPTPPAPM PGPTTGLVTG PDGKGMNPPS YYTQPAPIPN NNPITVQTVY VQHPITFLDR PIQMCCPSCN KMIVSQLSYN AGALTWLSCG SLCLLGCIAG CCFIPFCVDA LQDVDHYCPN CRALLGTYKR L

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LITAF His Protein

SDS-PAGE: Recombinant Human LITAF His Protein [NBP2-23200]

SDS-PAGE: Recombinant Human LITAF His Protein [NBP2-23200]

SDS-Page: Recombinant Human LITAF Protein [NBP2-23200]

Formulation, Preparation and Storage

NBP2-23200
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LITAF

Lipopolysaccharide-induced TNF-alpha factor, also known as LITAF, is a small integral membrane protein of lysosome/late endosome. LITAF mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. Recombinant human LITAF protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Lipopolysaccharide-induced TNF Factor

Alternate Names

PIG7, SIMPLE, TP53I7

Gene Symbol

LITAF

Additional LITAF Products

Product Documents for Recombinant Human LITAF His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human LITAF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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