Recombinant Human LMP7/PSMB8 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23374

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all LMP7/PSMB8 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 73-276 of Human LMP7/PSMB8

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTTTLA FKFQHGVIAA VDSRASAGSY ISALRVNKVI EINPYLLGTM SGCAADCQYW ERLLAKECRL YYLRNGERIS VSAASKLLSN MMCQYRGMGL SMGSMICGWD KKGPGLYYVD EHGTRLSGNM FSTGSGNTYA YGVMDSGYRP NLSPEEAYDL GRRAIAYATH RDSYSGGVVN MYHMKEDGWV KVESTDVSDL LHQYREANQ

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LMP7/PSMB8 His Protein

SDS-PAGE: Recombinant Human LMP7/PSMB8 His Protein [NBP2-23374]

SDS-PAGE: Recombinant Human LMP7/PSMB8 His Protein [NBP2-23374]

SDS-Page: Proteasome 20S LMP7 Protein [NBP2-23374]

Formulation, Preparation and Storage

NBP2-23374
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMP7/PSMB8

PSMB8, also known as LMP7, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB8 is located in the class II region of the MHC (major histocompatibility complex). Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. This protein acts as a major component of interferon gamma-induced sensitivity. It plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. Recombinant human PSMB8 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Large Multifunctional Protease 7

Alternate Names

ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i, PSMB8, RING10

Gene Symbol

PSMB8

Additional LMP7/PSMB8 Products

Product Documents for Recombinant Human LMP7/PSMB8 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human LMP7/PSMB8 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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