Can you provide us with a dilution this Peptide should be used at? Is their a recommendation concerning BSA or Milk powder blocking when using this peptide in combination with its appropriate antibody?

Name: LRRK2 Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NB300-268PEP

Our LRRK2 Peptide NB300-268PEP is designed to be used as a blocking peptide for NB300-268. We recommend using the peptide anywhere between 50-200 molar excess. We also recommend doing a titer to ensure proper blocking. We typically incubate the peptide with the antibody in PBS or TBS for 1 hour at room temperature with gentle shaking prior to incubation with the membrane. We have not noticed any interaction of the peptide with milk or BSA once antibody has been incubated with the peptide before incubating in a blocking buffer. Our blocking buffer contains 1% BSA, 5% non-fat dry milk in TBST.

LRRK2 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NB300-268PEP

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

Unconjugated

Applications

Antibody Competition

View all LRRK2 Proteins and Enzymes »

Product Specifications

Description

A LRRK2 antibody blocking peptide.

Application Notes

This peptide is useful as a blocking peptide for NB300-268. For further blocking peptide related protocol, click here.

Specificity

This peptide is specific for NB300-268 only.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation and Storage

NB300-268PEP

Formulation	Peptide dissolved in dH2O. Contains no BSA.
Preservative	No Preservative
Concentration	1.0 mg/ml
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: LRRK2

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Gene Symbol

LRRK2

Additional LRRK2 Products

Product Documents for LRRK2 Antibody Blocking Peptide

Product Datasheets

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COA

Product Specific Notices for LRRK2 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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