Recombinant Human MED4 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44479

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MED4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-270 of uman MED4

Source: E.coli

Amino Acid Sequence: MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MED4 His Protein

SDS-PAGE: Recombinant Human MED4 His Protein [NBP1-44479]

SDS-PAGE: Recombinant Human MED4 His Protein [NBP1-44479]

SDS-Page: Recombinant Human MED4 Protein [NBP1-44479] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-PAGE: Recombinant Human MED4 His Protein [NBP1-44479]

SDS-PAGE: Recombinant Human MED4 His Protein [NBP1-44479]

SDS-Page: Recombinant Human MED4 Protein [NBP1-44479] - MED4, 30.7kDa (278aa), confirmed by MALDI-TOF with a purity of 80% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-44479
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 100 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED4

Mediator complex subunit 4(MED4) is also known as mediator of RNA polymerase II transcription subunit 4 or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36). This protein is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner. Recombinant human MED4, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Mediator Complex Subunit 4

Alternate Names

ARC36, DRIP36, HSPC126, TRAP36, VDRIP

Gene Symbol

MED4

Additional MED4 Products

Product Documents for Recombinant Human MED4 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MED4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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