Recombinant Human Midkine Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34997
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 123 amino acids corresponding to Midkine Source: E. coli
Uniprot ID: P21741
Amino Acid Sequence: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of Midkine as determined by LAL method.
Predicted Molecular Mass
13.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Midkine Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Midkine Protein
![SDS-PAGE: Recombinant Human Midkine Protein [NBP2-34997]](https://resources.bio-techne.com/images/products/Human-Midkine-Protein-SDS-Page-NBP2-34997-img0001.jpg "SDS-PAGE: Recombinant Human Midkine Protein [NBP2-34997]")
SDS-PAGE: Recombinant Human Midkine Protein [NBP2-34997]
SDS-Page: Human Midkine Protein [NBP2-34997]Formulation, Preparation and Storage
NBP2-34997
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Midkine
Additional Midkine Products
Product Documents for Recombinant Human Midkine Protein
Product Specific Notices for Recombinant Human Midkine Protein
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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